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    The technique used to separate organelles is referred to as Ultracentrifugation or Subcellular Fractionation. It is used to extract undamaged organelles from tissue and separate them so they structure and Biochemistry can be studied in a greater detail under an electron microscope. The process starts with tissue, in this case leaf tissue first being sliced into fine pieces with scissors or a scalpel to decrease size. Fine strips of tissue will then be placed in a test
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    Fractionation. It is used to extract undamaged organelles from tissue and separate them so they structure and Biochemistry can be studied in a greater detail under an electron microscope. The process starts with tissue, in this case leaf tissue first being sliced into fine pieces with scissors or a scalpel to decrease size. Fine strips of tissue will then be placed in a tes
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    ey structure and Biochemistry can be studied in a greater detail under an electron microscope. The process starts with tissue, in this case leaf tissue first being sliced into fine pieces with scissors or a scalpel to decrease size. Fine strips of tissue will then be placed in a tes
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    The process starts with tissue, in this case leaf tissue first being sliced into fine pieces with scissors or a scalpel to decrease size. Fine strips of tissue will then be placed in a tes
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    ith scissors or a scalpel to decrease size. Fine strips of tissue will then be placed in a test tube.

    Cells contain enzymes (proteins) which are static in numerous parts of the cell, if the cell is to be burst these enzymes will start digesting the cell or organelles that they do not normally interact with, so an ice cold environment is used and an ice cold chemical solution called a buffer is added to the tissue, these in term decrease enzyme activity (enzymes

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